Identification of Iron Transporters Expressed in the Magnetotactic Bacterium Magnetospirillum magnetotacticum
نویسندگان
چکیده
منابع مشابه
Iron reductase for magnetite synthesis in the magnetotactic bacterium Magnetospirillum magnetotacticum.
Ferric iron reductase was purified from magnetotactic bacterium Magnetospirillum (formerly Aquaspirillum) magnetotacticum (ATCC 31632) to an electrophoretically homogeneous state. The enzyme was loosely bound on the cytoplasmic face of the cytoplasmic membrane and was found more frequently in magnetic cells than in nonmagnetic cells. The molecular mass of the purified enzyme was calculated upon...
متن کاملNitrate reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum MS-1: purification and sequence analyses.
We purified the nitrate reductase from the soluble fraction of Magnetospirillum magnetotacticum MS-1. The enzyme was composed of 86- and 17-kDa subunits and contained molybdenum, non-heme iron, and heme c. These properties are very similar to those of the periplasmic nitrate reductase found in Paracoccus pantotrophus. The M. magnetotacticum nap locus was clustered in seven open reading frames, ...
متن کاملEnzymatic Iron Oxidation and Reduction in Magnetite Synthesizing Magnetospirillum Magnetotacticum
We investigated the enzymatic reduction and oxidation of iron in M.magnetotacticum which synthesizes magnetite at room temperature. NADH-Fe(lI1) reductase with the molecular mass of 36kDa was purified from the bacterium. The enzyme was located in cytoplasm and utilized NADH and NADPH in the presence of FMN as reductant and showed maximum activity at pH 7.0. The Km for NADH and NADPH were about ...
متن کاملComplete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1.
Magnetospirillum sp. strain AMB-1 is a Gram-negative alpha-proteobacterium that synthesizes nano-sized magnetites, referred to as magnetosomes, aligned intracellularly in a chain. The potential of this nano-sized material is growing and will be applicable to broad research areas. It has been expected that genome analysis would elucidate the mechanism of magnetosome formation by magnetic bacteri...
متن کاملDevelopment of a cell surface display system in a magnetotactic bacterium, "Magnetospirillum magneticum" AMB-1.
Bacterial cell surface display is a widely used technology for bioadsorption and for the development of a variety of screening systems. Magnetotactic bacteria are unique species of bacteria due to the presence of magnetic nanoparticles within them. These intracellular, nanosized (50 to 100 nm) magnetic nanoparticles enable the cells to migrate and be manipulated by magnetic force. In this work,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Microbiology
سال: 2008
ISSN: 0343-8651,1432-0991
DOI: 10.1007/s00284-008-9305-7